Myh9 Polyclonal antibody

Myh9 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 51053-1-Ig

Synonyms

C80049, D0Jmb2, E030044M24Rik, Myh9, Myhn 1, Myhn1, TU72.6

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Validation Data Gallery

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  IHC staining of mouse kidney using 51053-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51053-1-Ig (Myh9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 51053-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51053-1-Ig (Myh9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

51053-1-Ig targets Myh9 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy polypeptide 9, non-muscle
• Calculated Molecular Weight: 226 kDa
• GenBank Accession Number: NM_022410
• Gene Symbol: Myh9
• Gene ID (NCBI): 17886
• RRID: AB_2147310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Mol Oncol; Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma; Authors - Mayu Nakagawa
human,mouse	WB	Cancer Biol Ther; Epigenetic silencing ZSCAN23 promotes pancreatic cancer growth by activating Wnt signaling; Authors - Qian Du