Tested Applications
| Positive WB detected in | 3T3-L1 cells, BV-2 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51132-1-AP targets Myocd in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myocardin |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | NM_146386 NM_145136 |
| Gene Symbol | Myocd |
| Gene ID (NCBI) | 214384 |
| RRID | AB_3670235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8VIM5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myocd (Myocardin) is involved in several processes, including circulatory system development; negative regulation of vascular-associated smooth muscle cell migration; and regulation of signal transduction. Located in chromatin and the nucleus. Orthologous to human MYOCD (myocardin).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Myocd antibody 51132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Myocd Polyclonal antibody (Cat# 51132-1-AP) has 6 total citations published in Biomaterials, Acta Pharmacologica Sinica, International Immunopharmacology (×2), American Journal of Physiology – Cell Physiology, and Journal of Diabetes and Metabolic Disorders. The research fields span cardiovascular biology, including arterial restenosis imaging, aortic dissection, erectile dysfunction, vascular calcification in chronic kidney disease, smooth muscle cell phenotypic switching, and Myocardin spatial expression in normal and disease states.
| Species | Application | Title |
|---|---|---|
Biomaterials Microenvironment-activatable nanoagent for real-time NIR-II monitoring and targeted therapy of arterial restenosis | ||
Int Immunopharmacol DLX2 drives vascular calcification in chronic kidney disease through glycolytic activation: a mechanism epigenetically regulated by HDAC2. | ||
Int Immunopharmacol Electrical stimulation improves arteriogenic erectile dysfunction by modulating CYLD-mediated macrophage-smooth muscle cell crosstalk. | ||
Am J Physiol Cell Physiol Spatial expression of Myocardin protein in normal and disease states. | ||
J Diabetes Metab Disord miR-148b attenuates ox-LDL-induced phenotypic switching of vascular smooth muscle cells by suppressing KLF4 expression and promoting its ubiquitination. |
