MYH2-specific Recombinant monoclonal antibody

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• MYH2-specific Recombinant monoclonal antibody (84099-1-RR)

MYH2-specific Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 84099-1-RR

Clone No.240810F8

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MYH2, Myosin 2, 240810F8, MyHC-2a, MyHC-IIa

• 

  IF Staining of C2C12 using 84099-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Myosin 2a antibody (84099-1-RR, Clone: 240810F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HSkMC using 84099-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using MYH2-specific antibody (84099-1-RR, Clone: 240810F8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Tested Applications

• Positive IF/ICC detected in: HSkMC cells, C2C12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

84099-1-RR targets MYH2-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, heavy chain 2, skeletal muscle, adult
• Calculated Molecular Weight: 223 kDa
• GenBank Accession Number: NM_017534
• Gene Symbol: MYH2
• Gene ID (NCBI): 4620
• RRID: AB_3671664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UKX2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Biochem Biophys Res Commun; Tamoxifen promotes myogenic differentiation and skeletal muscle regeneration via activation of the GRP94.; Authors - Lei Xing
	IF,WB	Food Res Int; Actin-myosin complex dissociation initiates programmed cell death during cold storage of grass carp muscle.; Authors - Xin Xiong