Testosterone ELISA Kit
Cat no : KE30009
Synonyms
17b hydroxy 4 androsten 3 one, T
Validation Data Gallery
Product Information
KE30009 is a vitro competitive binding enzyme-linked immunosorbent assay (Competitive ELISA). The testosterone ELISA kit is to be used to detect and quantify protein levels of vitro testosterone. A 96-well plate has been precoated with Testosterone antigen. Samples and the antibody-HRP conjugate are added to the wells, where testosterone in the sample competes with the testosterone antigen immobilized on the microplate for binding with the antibody-HRP. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 79% | 71%-90% |
| Urine | 94% | 82%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.69 | 5.87 | 2.77 |
| 2 | 8 | 1.51 | 0.43 | 0.24 |
| 3 | 8 | 6.12 | 7.33 | 8.66 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.95 | 6.21 | 2.96 |
| 2 | 16 | 1.99 | 0.60 | 0.32 |
| 3 | 16 | 7.67 | 9.66 | 10.81 |
Background Information
Testosterone is the primary male sex hormone (androgen) and is crucial for the development of male reproductive tissues, as well as for promoting secondary sexual characteristics. While it is produced in much larger amounts in men, it is also an important hormone for women, playing a key role in libido, bone strength, and muscle maintenance.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 17b hydroxy 4 androsten 3 one, T |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.