Testosterone ELISA Kit
Cat no : KE30009
Synonyms
17b hydroxy 4 androsten 3 one, T
Validation Data Gallery
Product Information
KE30009 is a vitro competitive binding enzyme-linked immunosorbent assay (Competitive ELISA). The testosterone ELISA kit is to be used to detect and quantify protein levels of vitro testosterone. A 96-well plate has been precoated with Testosterone antigen. Samples and the antibody-HRP conjugate are added to the wells, where testosterone in the sample competes with the testosterone antigen immobilized on the microplate for binding with the antibody-HRP. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 79% | 71%-90% |
| Urine | 94% | 82%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.69 | 5.87 | 2.77 |
| 2 | 8 | 1.51 | 0.43 | 0.24 |
| 3 | 8 | 6.12 | 7.33 | 8.66 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.95 | 6.21 | 2.96 |
| 2 | 16 | 1.99 | 0.60 | 0.32 |
| 3 | 16 | 7.67 | 9.66 | 10.81 |
Background Information
Testosterone is the primary male sex hormone (androgen) and is crucial for the development of male reproductive tissues, as well as for promoting secondary sexual characteristics. While it is produced in much larger amounts in men, it is also an important hormone for women, playing a key role in libido, bone strength, and muscle maintenance.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 17b hydroxy 4 androsten 3 one, T |