Tested Applications
| Positive WB detected in | HeLa cells, rat brain tissue, PC-3 cells, C2C12 cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30803-1-AP targets N-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | NM_001792.4 |
| Gene Symbol | N-cadherin |
| Gene ID (NCBI) | 1000 |
| RRID | AB_3669759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19022-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for N-cadherin antibody 30803-1-AP | Download protocol |
| IHC protocol for N-cadherin antibody 30803-1-AP | Download protocol |
| WB protocol for N-cadherin antibody 30803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The N-cadherin Polyclonal antibody (Cat# 30803-1-AP) has 3 total citations. These appear in Cancer Genetics, Molecular and Cellular Biochemistry, and Journal of Cell Science. The associated research fields include bladder cancer cell proliferation and migration (STING1 pathway), ferroptosis and tumor progression in triple-negative breast cancer (UCHL1/PARK7 axis), and cadherin junctional mechano-biology involving growth factor receptors.
| Species | Application | Title |
|---|---|---|
Cancer Genet Mechanistic study of Liquiritigenin inhibiting bladder cancer cell proliferation and migration by regulating STING1 | ||
Mol Cell Biochem UCHL1-mediated deubiquitination of PARK7 suppresses ferroptosis and enhances tumor progression in triple-negative breast cancer. | ||
J Cell Sci Cadherins and growth factor receptors - ligand-selective mechano-switches at cadherin junctions. |
