N-cadherin Polyclonal antibody

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  WB analysis using 30803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30803-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 30803-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30803-1-AP (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 30803-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 30803-1-AP (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 30803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using N-cadherin antibody (30803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

N-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30803-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

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Tested Applications

• Positive WB detected in: HeLa cells, rat brain tissue, PC-3 cells, C2C12 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30803-1-AP targets N-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001792.4
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• RRID: AB_3669759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19022-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Genet; Mechanistic study of Liquiritigenin inhibiting bladder cancer cell proliferation and migration by regulating STING1; Authors - Wuheng Li