NAB1 Polyclonal antibody

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• NAB1 Polyclonal antibody (18541-1-AP)

NAB1 Polyclonal Antibody for WB, ELISA

Cat No. 18541-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EGR 1 binding protein 1, NAB1, NGFI A binding protein 1

• 

  WB analysis of HepG2 using 18541-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18541-1-AP (NAB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 18541-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18541-1-AP (NAB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18541-1-AP targets NAB1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13407

  Product name: Recombinant human NAB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 135-487 aa of BC035724

  Sequence: CVQSLGQGKSDVVGSLALQSVGESRLWQGHHATESEHSLSPADLGSPASPKESSEALDAAAALSVAECVERMAPTLPKSDLNEVKELLKTNKKLAKMIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISREVTYKYTYRTTKSKCGERDELSPKRIKVEDGFPDFQDSVQTLFQQARAKSEELAALSSQQPEKVMAKQMEFLCNQAGYERLQHAERRLSAGLYRQSSEEHSPNGLTSDNSDGQGERPLNLRMPNLQNRQPHHFVVDGELSRLYPSEAKSHSSESLGILKDYPHSAFTLEKKVIKTEPEDSR

• Full Name: NGFI-A binding protein 1 (EGR1 binding protein 1)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC035724
• Gene Symbol: NAB1
• Gene ID (NCBI): 4664
• RRID: AB_2878551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13506
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

1.0 out of 5

1 verified customer reviews

What customers say

The single review for this product was negative (1 star). The customer used it for Western Blot at a 1:600 dilution on murine liver cell protein and reported that the antibody failed to produce a band at the expected molecular weight, while also generating non-specific signal. Overall, the feedback indicates poor performance in this application, with both lack of specific detection and background issues.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Carmalena (Verified Customer) (08-06-2026)

Did not output a band where expected. Also non-specific signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: Nuclear/Cytosolic/Total protein of murine liver cells

NAB1 Antibody Western Blot validation (1:600 dilution) in Nuclear/Cytosolic/Total protein of murine liver cells (Cat no:18541-1-AP)