Tested Applications
| Positive WB detected in | HEK-293 cells, Hela cells, Neuro-2a cells, NIH/3T3 cells, rat kidney tissue |
| Positive IHC detected in | human ovary cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29290-1-AP targets NADK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30982 Product name: Recombinant human NADK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-446 aa of BC001709 Sequence: SRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG Predict reactive species |
| Full Name | NAD kinase |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC001709 |
| Gene Symbol | NADK |
| Gene ID (NCBI) | 65220 |
| RRID | AB_2918272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95544 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NADK antibody 29290-1-AP | Download protocol |
| WB protocol for NADK antibody 29290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NADK polyclonal antibody (Cat# 29290-1-AP) has 3 citations. These appear in Nature Communications, Antioxidants (Basel), and Nature Metabolism. The associated research fields include vascular aging and endothelial NADPH metabolism, ferroptosis susceptibility governed by NADPH homeostasis, and cardiac physiological hypertrophy with ischemia-reperfusion injury involving pentose phosphate pathway–derived NADPH. All cited applications are Western blot in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging. | ||
Antioxidants (Basel) NADK Governs Ferroptosis Susceptibility by Orchestrating NADPH Homeostasis.
| ||
Nat Metab Pentose phosphate pathway-derived NADPH facilitates physiological hypertrophy and alleviates ischemia-reperfusion injury in the heart. |
