Nanog Recombinant antibody, PBS Only (Capture/Detector)

• 

  WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02157-2

  Sandwich ELISA standard curve of MP02157-2, Human Nanog Recombinant Matched Antibody Pair - PBS only. 80210-13-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5645. 80210-12-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP02157-2

  The mean Nanog concentration was determined to be 9.25 ng/mL in NCCIT cell extract based on a 1.50 mg/mL extract load.

• 

  IF Staining of NCCIT using 80210-13-RR (same clone as 80210-13-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Nanog antibody (80210-13-RR, Clone: 250067F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02157-2

  Cytometric bead array standard curve of MP02157-2, Nanog Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80210-13-PBS. Detection antibody: 80210-12-PBS. Standard: Ag5645. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 80210-13-RR

  Biolayer interferometry (BLl) kinetic assays of 80210-13-RR against Human Nanog were performed. The affinity constant is 11.0 nM.

Nanog Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 80210-13-PBS

Clone No.250067F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

hNanog

---

Product Information

80210-13-PBS targets Nanog as part of a matched antibody pair:

MP02157-1: 80210-11-PBS capture and 80210-13-PBS detection (validated in Cytometric bead array)

MP02157-2: 80210-13-PBS capture and 80210-12-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Nanog fusion protein Ag5645
• Full Name: Nanog homeobox
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC160187
• Gene Symbol: Nanog
• Gene ID (NCBI): 79923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H9S0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)