Tested Applications
| Positive WB detected in | mouse testis tissue, HEK-293 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21679-1-AP targets NANOS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16014 Product name: Recombinant human NANOS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC101209 Sequence: MGTFDLWTDYLGLAHLVRALSGKEGPETRLSPQPEPEPMLEPVSALEPMPAPESVPVPGPKDQKRSLESSPAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATRERAHTRRFCPLTGQGYTSVYSHTTRNSAGKKLVRPDKAKTQDTGHRRGGGGGAGFRGAGKSEPSPSCSPSMST Predict reactive species |
| Full Name | nanos homolog 3 (Drosophila) |
| Calculated Molecular Weight | 192 aa, 21 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC101209 |
| Gene Symbol | NANOS3 |
| Gene ID (NCBI) | 342977 |
| RRID | AB_10859250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60323 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NANOS3 antibody 21679-1-AP | Download protocol |
| WB protocol for NANOS3 antibody 21679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-NANOS3 (Cat# 21679-1-AP) has 8 total citations published in Nucleic Acids Research, Stem Cell Research & Therapy, Environmental Pollution, Journal of Ethnopharmacology, Journal of Pathology, BMC Cancer, Experimental Cell Research, and Biology of Reproduction. Research fields span germline specification, ovarian aging, stem cell differentiation, cardiac hypertrophy, lung tumour invasion via EMT, cardiomyocyte apoptosis, and spermatogenesis.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Regulation of both transcription and RNA turnover contribute to germline specification. | ||
Stem Cell Res Ther CNOT3 resists ovarian aging by accelerating oocyte maturation and promoting ESCs differentiation. | ||
Environ Pollut Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling | ||
J Ethnopharmacol Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC (soluble guanylate cyclase) - cGMP (cyclic guanosine monophosphate) - PKG (protein kinase G) pathway | ||
J Pathol The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition. | ||
