Alpha SNAP Polyclonal antibody

Alpha SNAP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10546-1-AP

Synonyms

alpha-SNAP, NAPA, SNAP alpha, SNAPA

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Validation Data Gallery

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  WB analysis using 10546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10546-1-AP (alpha-SNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10546-1-AP (Alpha SNAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10546-1-AP (Alpha SNAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10546-1-AP targets Alpha SNAP in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha SNAP fusion protein Ag0836
• Full Name: N-ethylmaleimide-sensitive factor attachment protein, alpha
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC007432
• Gene Symbol: NAPA
• Gene ID (NCBI): 8775
• RRID: AB_2150721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAPs (soluble NSF-attachment proteins) may play an important role in the process of docking and fusion of vesicles to their target membranes, according to the 'SNARE hypothesis'. NAPA(Alpha- SNAP) was found in a wide range of tissues and act synergistically in intra-Golgi transport. This antibody recognizes a 33kd protein in SDS page.