NAPG Polyclonal antibody

NAPG Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15604-1-AP

Synonyms

GAMMASNAP, NAPG, SNAP gamma, SNAPG

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Validation Data Gallery

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  WB analysis using 15604-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15604-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15604-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15604-1-AP

  IP result of anti-NAPG (IP:15604-1-AP, 4ug; Detection:15604-1-AP 1:400) with HepG2 cells lysate 3300 ug.

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  IHC staining of human ovary tumor using 15604-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15604-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15604-1-AP (NAPG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A375 cells, K-562 cells, HepG2 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15604-1-AP targets NAPG in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAPG fusion protein Ag7988
• Full Name: N-ethylmaleimide-sensitive factor attachment protein, gamma
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001889
• Gene Symbol: NAPG
• Gene ID (NCBI): 8774
• RRID: AB_2150885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.