Napsin A Recombinant monoclonal antibody

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• Napsin A Recombinant monoclonal antibody (86052-4-RR)

Napsin A Uni-rAb^® Recombinant Antibody for IHC, IF-P, ELISA

Cat No. 86052-4-RR

Clone No.242433C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Synonyms

NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-

• 

  IHC staining of human kidney using 86052-4-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 86052-4-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 86052-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 86052-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human kidney using 86052-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human kidney using 86052-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human lung tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86052-4-RR targets Napsin A in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9721

  Product name: Recombinant human NAPSA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-420 aa of BC017842

  Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG

• Full Name: napsin A aspartic peptidase
• Calculated Molecular Weight: 420 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC017842
• Gene Symbol: Napsin A
• Gene ID (NCBI): 9476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O96009
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.