NARF Polyclonal antibody

NARF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12173-1-AP

Synonyms

IOP2, NARF

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Validation Data Gallery

• 

  WB analysis of human liver using 12173-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12173-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12173-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12173-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human liver tissue, human lung tissue, human placenta tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12173-1-AP targets NARF in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NARF fusion protein Ag2816
• Full Name: nuclear prelamin A recognition factor
• Calculated Molecular Weight: 46 kDa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC016440
• Gene Symbol: NARF
• Gene ID (NCBI): 26502
• RRID: AB_2151027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prelamin A, is processed in the nucleus to lamin A by removal of its final 18 amino acids, including the cysteine residue in its C-terminal CAAX box, which is farnesylated. Nuclear prelamin A recognition factor (NARF) binds the farnesylated prelamin A C-terminal domain(preAct)(PMID:10514485). NARF, at 52 kDa, also named as IOP2, is a small enough protein to freely diffuse through the nuclear pore complex and may be retained within the nucleus by binding nuclear proteins and it has all of the information necessary to be imported into the nucleus(PMID:10514485). It has 3 isoforms produced by alternative splicing with the molecular mass of 51 kDa, 56 kDa and 46 kDa,respectively.