NARG1L Polyclonal antibody

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  WB analysis of Jurkat using 17427-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17427-1-AP (NARG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17427-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17427-1-AP (NARG1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 17427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17427-1-AP (NARG1L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 17427-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NARG1L antibody (17427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NARG1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17427-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NAA16, N-alpha-acetyltransferase 16, NatA auxiliary subunit, NARG1 like protein, NARG1-like protein, NAT2

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17427-1-AP targets NARG1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11492

  Product name: Recombinant human NARG1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 154-501 aa of BC028112

  Sequence: EITDDQFDFHTYCMRKMTLRAYVDLLRLEDILRRHAFYFKAARSAIEIYLKLYDNPLTNESKQQEINSENLSAKELKKMLSKQRRAQKKAKLEEERKHAERERQQKNQKKKRDEEEEEASGLKEELIPEKLERVENPLEEAVKFLIPLKNLVADNIDTHLLAFEIYFRKGKFLLMLQSVKRAFAINSNNPWLHECLIRFSKSVSNHSNLPDIVSKVLSQEMQKIFVKKDLESFNEDFLKRNATSLQHLLSGAKMMYFLDKSRQEKAIAIATRLDETIKDKDVKTLIKVSEALLDGSFGNCSSQYEEYRMACHNLLPFTSAFLPAVNEVDNPNVALNHTANYDVLANEI

• Full Name: NMDA receptor regulated 1-like
• Calculated Molecular Weight: 864 aa, 101 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC028112
• Gene Symbol: NARG1L
• Gene ID (NCBI): 79612
• RRID: AB_1939557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6N069
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMDA receptor-regulated 1-like protein (NARG1L, also known as NAA16), is the auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity.