NARG1L Recombinant monoclonal antibody

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  WB analysis using 85860-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85860-4-RR (NARG1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85860-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85860-4-RR against Human NARG1L were performed. The affinity constant is below 1 pM.

NARG1L Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85860-4-RR

Clone No.250181F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NAA16, N-alpha-acetyltransferase 16, NatA auxiliary subunit, NARG1 like protein, NARG1-like protein, NAT2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, A549 cells, SH-SY5Y cells, mouse spleen tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85860-4-RR targets NARG1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11492

  Product name: Recombinant human NARG1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 154-501 aa of BC028112

  Sequence: EITDDQFDFHTYCMRKMTLRAYVDLLRLEDILRRHAFYFKAARSAIEIYLKLYDNPLTNESKQQEINSENLSAKELKKMLSKQRRAQKKAKLEEERKHAERERQQKNQKKKRDEEEEEASGLKEELIPEKLERVENPLEEAVKFLIPLKNLVADNIDTHLLAFEIYFRKGKFLLMLQSVKRAFAINSNNPWLHECLIRFSKSVSNHSNLPDIVSKVLSQEMQKIFVKKDLESFNEDFLKRNATSLQHLLSGAKMMYFLDKSRQEKAIAIATRLDETIKDKDVKTLIKVSEALLDGSFGNCSSQYEEYRMACHNLLPFTSAFLPAVNEVDNPNVALNHTANYDVLANEI

• Full Name: NMDA receptor regulated 1-like
• Calculated Molecular Weight: 864 aa, 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC028112
• Gene Symbol: NARG1L
• Gene ID (NCBI): 79612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6N069
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMDA receptor-regulated 1-like protein (NARG1L, also known as NAA16), is the auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity.