NARS Polyclonal antibody

NARS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14882-1-AP

Synonyms

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

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Validation Data Gallery

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  WB analysis using 14882-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14882-1-AP (NARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14882-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14882-1-AP (NARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14882-1-AP

  IP result of anti-NARS (IP:14882-1-AP, 4ug; Detection:14882-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver cancer using 14882-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14882-1-AP (NARS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14882-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14882-1-AP (NARS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14882-1-AP targets NARS in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NARS fusion protein Ag6656
• Full Name: asparaginyl-tRNA synthetase
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC001687
• Gene Symbol: NARS
• Gene ID (NCBI): 4677
• RRID: AB_2151040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

Publications

Species	Application	Title
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj