NARS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15714-1-AP

Synonyms

AsnRS, Asparagine tRNA ligase, NARS2, SLM5

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Validation Data Gallery

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  WB analysis using 15714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15714-1-AP

  WB result of NARS2 antibody (15714-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NARS2 transfected HepG2 cells.

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  WB analysis of human liver using 15714-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15714-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15714-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, human brain tissue, K-562 cells, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, human lung tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15714-1-AP targets NARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NARS2 fusion protein Ag8345
• Full Name: asparaginyl-tRNA synthetase 2, mitochondrial (putative)
• Calculated Molecular Weight: 477 aa, 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC007800
• Gene Symbol: NARS2
• Gene ID (NCBI): 79731
• RRID: AB_2151157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.