Tested Applications
| Positive WB detected in | HepG2 cells, human liver tissue, human brain tissue, K-562 cells, mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue, human lung tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15714-1-AP targets NARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8345 Product name: Recombinant human NARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-477 aa of BC007800 Sequence: ERHPLEYLRQYPHFRCRTNVLGSILRIRSEATAAIHSFFKDSGFVHIHTPIITSNDSEGAGELFQLEPSGKLKVPEENFFNVPAFLTVSGQLHLEVMSGAFTQVFTFGPTFRAENSQSRRHLAEFYMIEAEISFVDSLQDLMQVIEELFKATTMMVLSKCPEDVELCHKFIAPGQKDRLEHMLKNNFLIISYTEAVEILKQASQNFTFTPEWGADLRTEHEKYLVKHCGNIPVFVINYPLTLKPFYMRDNEDGPQHTVAAVDLLVPGVGELFGGGLREERYHFLEERLARSGLTEVYQWYLDLRRFGSVPHGGFGMGFERYLQCILGVDNIKDVIPFPRFPHSCLL Predict reactive species |
| Full Name | asparaginyl-tRNA synthetase 2, mitochondrial (putative) |
| Calculated Molecular Weight | 477 aa, 54 kDa |
| Observed Molecular Weight | 50-54 kDa |
| GenBank Accession Number | BC007800 |
| Gene Symbol | NARS2 |
| Gene ID (NCBI) | 79731 |
| RRID | AB_2151157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96I59 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NARS2 (asparaginyl-tRNA synthetase 2, mitochondrial) is a nuclear-encoded enzyme that functions within the mitochondria, the energy-producing organelles of the cell. It belongs to the class II family of aminoacyl-tRNA synthetases (aaRSs), a group of essential enzymes responsible for protein synthesis. Its proper function is indispensable for mitochondrial protein synthesis and energy production, and its deficiency underlies a spectrum of severe human metabolic and neurological diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NARS2 antibody 15714-1-AP | Download protocol |
| IHC protocol for NARS2 antibody 15714-1-AP | Download protocol |
| WB protocol for NARS2 antibody 15714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NARS2 Polyclonal antibody (Cat# 15714-1-AP) has 2 total citations. These appear in Cell Reports (2025) and EMBO Molecular Medicine (2026). The research fields covered include mitochondrial membrane potential regulation in leukemic cells and comprehensive functional testing in fibroblasts for diagnosing mitochondrial disease.
| Species | Application | Title |
|---|---|---|
Cell Rep A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential.
| ||
EMBO Mol Med Comprehensive functional testing in fibroblasts has strong utility to diagnose mitochondrial disease. |
Reviews
What customers say
One reviewer (Vera S.) tested this antibody by Western Blot on fibroblasts at a 1:1000 dilution and rated it 3 out of 5. They reported that binding was quite low at that dilution, though the antibody did work. They also noted some unspecific binding to the ladder. Overall, the feedback suggests the antibody is functional but may benefit from optimization of dilution to improve signal strength and reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vera (Verified Customer) (07-14-2026) | Antibody shows quite low binding at a dilution of 1:1000, but it does work. Antibody also shows some unspecific binding to the ladder.
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