NASP Polyclonal antibody

• Featured Product
• KD/KO Validated

NASP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11323-1-AP

Synonyms

PRO1999, FLB7527

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Validation Data Gallery

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  WB analysis of mouse testis using 11323-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11323-1-AP (NASP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11323-1-AP

  IP result of anti-NASP (IP:11323-1-AP, 5ug; Detection:11323-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11323-1-AP (NASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11323-1-AP (NASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lymphoma using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11323-1-AP (NASP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11323-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NASP antibody 11323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

11323-1-AP targets NASP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NASP fusion protein Ag1824
• Full Name: nuclear autoantigenic sperm protein (histone-binding)
• Calculated Molecular Weight: 788 aa, 85 kDa
• Observed Molecular Weight: 150 kDa, 70 kDa
• GenBank Accession Number: BC010105
• Gene Symbol: NASP
• Gene ID (NCBI): 4678
• RRID: AB_2151166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.

Publications

Species	Application	Title
human,mouse	WB	Genes Dev; The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.; Authors - Drané Pascal P
human	WB	Nat Struct Mol Biol; The program for processing newly synthesized histones H3.1 and H4.; Authors - Campos Eric I EI ; KD Validated
human	WB	J Cell Sci; Role of Template Activating Factor-I as a chaperone in linker histone dynamics.; Authors - Kato Kohsuke K
human,mouse,rat	WB,IHC	J Biol Chem; MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP).; Authors - Ma Wubin W
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
human	WB	Int J Nanomedicine; Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity.; Authors - Qing Yuan