NASP Monoclonal antibody

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  WB analysis using 68710-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68710-1-Ig (NASP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

NASP Monoclonal Antibody for WB, ELISA

Cat No. 68710-1-Ig

Clone No.1A1A1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp547F162, FLB7527, FLJ31599, FLJ35510, NASP, PRO1999

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Ramos cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68710-1-Ig targets NASP in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1824

  Product name: Recombinant human NASP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 439-788 aa of BC010105

  Sequence: VDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC

• Full Name: nuclear autoantigenic sperm protein (histone-binding)
• Calculated Molecular Weight: 788 aa, 85 kDa
• Observed Molecular Weight: 150 kDa, 70 kDa
• GenBank Accession Number: BC010105
• Gene Symbol: NASP
• Gene ID (NCBI): 4678
• RRID: AB_3670403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49321
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.