NAT5 Polyclonal antibody

NAT5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15807-1-AP

Synonyms

N-alpha-acetyltransferase 20, N-acetyltransferase 5, NAA20, N acetyltransferase 5, Methionine N-acetyltransferase

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Validation Data Gallery

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  WB analysis of HeLa using 15807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15807-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 15807-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15807-1-AP (NAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 15807-1-AP

  IP result of anti-NAT5 (IP:15807-1-AP, 4ug; Detection:15807-1-AP 1:500) with HL-60 cells lysate 3040ug.

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  IHC staining of human ovary cancer using 15807-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15807-1-AP (NAT5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15807-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15807-1-AP (NAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, human brain tissue
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15807-1-AP targets NAT5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAT5 fusion protein Ag8541
• Full Name: N-acetyltransferase 5 (GCN5-related, putative)
• Calculated Molecular Weight: 178 aa, 20 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC005181
• Gene Symbol: NAT5
• Gene ID (NCBI): 51126
• RRID: AB_1640056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; NatB Catalytic Subunit Depletion Disrupts DNA Replication Initiation Leading to Senescence in MEFs; Authors - Jasmin Elurbide
human	WB	Oncotarget; NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma.; Authors - Leire Neri
mouse		Mol Cell Biol; NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway; Authors - Joana P Guedes