Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14683-1-AP targets NBAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6407 Product name: Recombinant human NBAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2017-2371 aa of BC051792 Sequence: MIQQLLEVAVGPLDISPKDIVQSAIMKIISALSGGSADLGGPRDPLKVLEGVVAAVHTSVDKGEELVSPEDLLEWLRPFCADDAWPVRPRIHVLQILGQSFHLTEEDSKLLVFFRTEAILKASWPQRQVDIADIENEENRYCLFMELLESSHHEAEFQHLVLLLQAWPPMKSEYVITNNPWVRLATVMLTRCTMENKEGLGNEVLKMCRSLYNTKQMLPAEGVKELCLLLLNQSLLLPSLKLLLESRDEHLHEMALEQITAVTTVNDSNCDQELLSLLLDAKLLVKCVSTPFYPRIVDHLLASLQQGRWDAEELGRHLREAGHEAEAGSLLLAVRGTHQAFRTFSTALRAAQHWV Predict reactive species |
| Full Name | neuroblastoma amplified sequence |
| Calculated Molecular Weight | 269 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC051792 |
| Gene Symbol | NBAS |
| Gene ID (NCBI) | 51594 |
| RRID | AB_1959590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A2RRP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NBAS, also known as neuroblastoma amplified (NAG) gene, was identified as a gene that is co-amplified with the N-myc (MYCN) gene in neuroblastoma cell lines. NBAS is a factor of the nonsense-mediated mRNA decay (NMD), which is a highly conserved surveillance pathway that allows the organism to respond to cellular and environmental stress.(PMID: 23828042)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NBAS antibody 14683-1-AP | Download protocol |
| IHC protocol for NBAS antibody 14683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NBAS Polyclonal antibody (Cat# 14683-1-AP) has 2 citations. One appears in J Biol Chem (2023), studying RAB18-mediated sterol mobilization and biosynthesis via proximity biotinylation in human cells (IF application). The other is in Bone (2018), investigating NBAS mutations in a family with Acrofrontofacionasal Dysostosis type 1 (WB application). The associated research fields are lipid metabolism/sterol biosynthesis and genetic skeletal dysplasia.
| Species | Application | Title |
|---|---|---|
J Biol Chem Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis | ||
Bone Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1. |
Reviews
What customers say
The single available review for this product (rated 1 out of 5) was from a Western Blot experiment on MEF cells. The reviewer noted that the antibody detected two bands at approximately 150 kD and 100 kD, which did not match the expected molecular weight of over 250 kD, suggesting potential issues with specificity or recognition of the intended target.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (10-27-2021) | This antibody primarily recognised two bands around 150 and 100 kd. The calculated molecular weight is over 250 kd.
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