NBAS Polyclonal antibody

NBAS Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14683-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Neuroblastoma-amplified sequence, Neuroblastoma-amplified gene protein, NBAS subunit of NRZ tethering complex, NAG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14683-1-AP targets NBAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6407

Product name: Recombinant human NBAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2017-2371 aa of BC051792

Sequence: MIQQLLEVAVGPLDISPKDIVQSAIMKIISALSGGSADLGGPRDPLKVLEGVVAAVHTSVDKGEELVSPEDLLEWLRPFCADDAWPVRPRIHVLQILGQSFHLTEEDSKLLVFFRTEAILKASWPQRQVDIADIENEENRYCLFMELLESSHHEAEFQHLVLLLQAWPPMKSEYVITNNPWVRLATVMLTRCTMENKEGLGNEVLKMCRSLYNTKQMLPAEGVKELCLLLLNQSLLLPSLKLLLESRDEHLHEMALEQITAVTTVNDSNCDQELLSLLLDAKLLVKCVSTPFYPRIVDHLLASLQQGRWDAEELGRHLREAGHEAEAGSLLLAVRGTHQAFRTFSTALRAAQHWV

Predict reactive species
Full Name neuroblastoma amplified sequence
Calculated Molecular Weight 269 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC051792
Gene Symbol NBAS
Gene ID (NCBI) 51594
RRIDAB_1959590
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA2RRP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBAS, also known as neuroblastoma amplified (NAG) gene, was identified as a gene that is co-amplified with the N-myc (MYCN) gene in neuroblastoma cell lines. NBAS is a factor of the nonsense-mediated mRNA decay (NMD), which is a highly conserved surveillance pathway that allows the organism to respond to cellular and environmental stress.(PMID: 23828042)

Protocols

Product Specific Protocols
IHC protocol for NBAS antibody 14683-1-APDownload protocol
IF protocol for NBAS antibody 14683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bone

Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1.

Authors - Eleonora Palagano
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (10-27-2021)

This antibody primarily recognised two bands around 150 and 100 kd. The calculated molecular weight is over 250 kd.

  • Applications: Western Blot
  • Cell Tissue Type: MEF
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