Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
14683-1-AP targets NBAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6407 Product name: Recombinant human NBAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2017-2371 aa of BC051792 Sequence: MIQQLLEVAVGPLDISPKDIVQSAIMKIISALSGGSADLGGPRDPLKVLEGVVAAVHTSVDKGEELVSPEDLLEWLRPFCADDAWPVRPRIHVLQILGQSFHLTEEDSKLLVFFRTEAILKASWPQRQVDIADIENEENRYCLFMELLESSHHEAEFQHLVLLLQAWPPMKSEYVITNNPWVRLATVMLTRCTMENKEGLGNEVLKMCRSLYNTKQMLPAEGVKELCLLLLNQSLLLPSLKLLLESRDEHLHEMALEQITAVTTVNDSNCDQELLSLLLDAKLLVKCVSTPFYPRIVDHLLASLQQGRWDAEELGRHLREAGHEAEAGSLLLAVRGTHQAFRTFSTALRAAQHWV Predict reactive species |
| Full Name | neuroblastoma amplified sequence |
| Calculated Molecular Weight | 269 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC051792 |
| Gene Symbol | NBAS |
| Gene ID (NCBI) | 51594 |
| RRID | AB_1959590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A2RRP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NBAS, also known as neuroblastoma amplified (NAG) gene, was identified as a gene that is co-amplified with the N-myc (MYCN) gene in neuroblastoma cell lines. NBAS is a factor of the nonsense-mediated mRNA decay (NMD), which is a highly conserved surveillance pathway that allows the organism to respond to cellular and environmental stress.(PMID: 23828042)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NBAS antibody 14683-1-AP | Download protocol |
| IHC protocol for NBAS antibody 14683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bone Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1. | ||
J Biol Chem Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (10-27-2021) | This antibody primarily recognised two bands around 150 and 100 kd. The calculated molecular weight is over 250 kd.
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