NCAM2 Polyclonal antibody

NCAM2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13850-1-AP

Synonyms

N CAM 2, NCAM 2, NCAM2, NCAM21



Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13850-1-AP targets NCAM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCAM2 fusion protein Ag4821
Full Name neural cell adhesion molecule 2
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC052946
Gene Symbol NCAM2
Gene ID (NCBI) 4685
RRIDAB_2237029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NCAM2 antibody 13850-1-APDownload protocol
IHC protocol for NCAM2 antibody 13850-1-APDownload protocol
IP protocol for NCAM2 antibody 13850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Comp Neurol

Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development

Authors - Enrico Amato
mouseIHC

bioRxiv

Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development

Authors - Enrico Amato