NCAPG Polyclonal antibody

• Featured Product
• KD/KO Validated

NCAPG Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 24563-1-AP

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

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Validation Data Gallery

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  WB analysis using 24563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24563-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24563-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24563-1-AP

  IP result of anti-NCAPG (IP:24563-1-AP, 4ug; Detection:24563-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

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  IHC staining of human testis using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 24563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 24563-1-AP

  Immunofluorescent analysis of HeLa cells using 24563-1-AP (NCAPG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 24563-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human testis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24563-1-AP is NCAPG Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCAPG fusion protein Ag19926
• Full Name: non-SMC condensin I complex, subunit G
• Calculated Molecular Weight: 1015 aa, 114 kDa
• Observed Molecular Weight: 131 kDa
• GenBank Accession Number: BC068467
• Gene Symbol: NCAPG
• Gene ID (NCBI): 64151
• RRID: AB_2879610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
human	WB	Front Oncol; NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma.; Authors - Guangrong Zheng ; KD Validated
human,mouse	WB,IHC,CoIP	J Transl Med; NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.; Authors - Rongguiyi Zhang ; KD Validated
human	WB	J Gastrointest Oncol; NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer; Authors - Hong Yang ; KD Validated
human	WB,IHC	Cell Death Dis; NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer.; Authors - Lili Jiang ; KD Validated
human	IHC	Sci Rep; Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.; Authors - Yoshihiro Miyazaki