NCAPH2 Polyclonal antibody

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• NCAPH2 Polyclonal antibody (26172-1-AP)

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• KD/KO Validated

NCAPH2 Polyclonal Antibody for WB, ELISA

Cat No. 26172-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

384D8 2, 384D8_6, CAP H2, CAPH2, Condensin 2 complex subunit H2, hCAP H2, Kleisin beta, NCAPH2

• 

  WB analysis using 26172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26172-1-AP (NCAPH2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 26172-1-AP

  WB result of NCAPH2 antibody (26172-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH2 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26172-1-AP targets NCAPH2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23134

  Product name: Recombinant human NCAPH2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC001298

  Sequence: MEDVEARFAHLLQPIRDLTKNWEVDVAAQLGEYLEELDQICISFDEGKTTMNFIEAALLIQGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSSVQEDRANGVASSGVPQEAENEFLSLDDFPDSRTNVDLKNDQTPSEVLIIPLLPMALVAPDEMEKNNNPLYSRQGEVLASRKDFRMNTCVPHPRGAFMLEPEGMSPMEPAGVSPMPGTQKDTGRTEEQPMEVSVCRSPVPALGFSQEPGPSPEGPMPLGGGEDEDAEEAVELPEASAPKAALEPKESRSPQQSAALPRRYMLREREGAPEPASCVKETPDPWQSLDPFDSLESKPFKKGRPYSVPPCVEEALGQK

• Full Name: non-SMC condensin II complex, subunit H2
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC001298
• Gene Symbol: NCAPH2
• Gene ID (NCBI): 29781
• RRID: AB_2880411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6IBW4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The NCAPH2 Polyclonal antibody (Cat# 26172-1-AP) has 2 citations. These appear in bioRxiv (a preprint on genome-wide DNA supercoiling and transcriptional feedback) and eLife (a study on human eIF2A in translation initiation). Both citations use the antibody for Western blot in human samples, spanning research fields of chromatin biology/transcription regulation and translational control.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	bioRxiv; Genome-wide DNA supercoiling arises from transcription and SMC activity and mediates transcriptional negative feedback.; Authors - Linying Zhu
human	WB	Elife; Human eIF2A has a minimal role in translation initiation and in uORF-mediated translational control in HeLa cells; Authors - Mykola Roiuk