NCAPH2 Polyclonal antibody

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  WB analysis using 26172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26172-1-AP (NCAPH2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26172-1-AP

  WB result of NCAPH2 antibody (26172-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH2 transfected MCF-7 cells.

• Featured Product
• KD/KO Validated

NCAPH2 Polyclonal Antibody for WB, ELISA

Cat No. 26172-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

384D8 2, 384D8_6, CAP H2, CAPH2, Condensin 2 complex subunit H2, hCAP H2, Kleisin beta, NCAPH2

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26172-1-AP targets NCAPH2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23134

  Product name: Recombinant human NCAPH2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC001298

  Sequence: MEDVEARFAHLLQPIRDLTKNWEVDVAAQLGEYLEELDQICISFDEGKTTMNFIEAALLIQGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSSVQEDRANGVASSGVPQEAENEFLSLDDFPDSRTNVDLKNDQTPSEVLIIPLLPMALVAPDEMEKNNNPLYSRQGEVLASRKDFRMNTCVPHPRGAFMLEPEGMSPMEPAGVSPMPGTQKDTGRTEEQPMEVSVCRSPVPALGFSQEPGPSPEGPMPLGGGEDEDAEEAVELPEASAPKAALEPKESRSPQQSAALPRRYMLREREGAPEPASCVKETPDPWQSLDPFDSLESKPFKKGRPYSVPPCVEEALGQK

• Full Name: non-SMC condensin II complex, subunit H2
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC001298
• Gene Symbol: NCAPH2
• Gene ID (NCBI): 29781
• RRID: AB_2880411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6IBW4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.