NOXA2/p67phox Polyclonal antibody

NOXA2/p67phox Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15551-1-AP

Synonyms

NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, p67phox



Tested Applications

Positive WB detected inCOLO 320 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15551-1-AP targets NOXA2/p67phox in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOXA2/p67phox fusion protein Ag7909
Full Name neutrophil cytosolic factor 2
Calculated molecular weight 60 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC001606
Gene symbol NCF2
Gene ID (NCBI) 4688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NOXA2/p67phox antibody 15551-1-APDownload protocol
IHC protocol for NOXA2/p67phox antibody 15551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuropharmacology

PMID: 30710569

Authors - Tianyi Wang
ratWB

J Physiol

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

Authors - C E Aiken
ratWB

Oxid Med Cell Longev

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.

Authors - Mengyang Zhang
ratWB

Andrologia

PMID: 30311248

Authors - Kai Cui
ratWB

Asian J Androl

Lipoxin A4 improves erectile dysfunction in rats with type I diabetes by inhibiting oxidative stress and corporal fibrosis.

Authors - Kai Cui
mouseIHC

Nutrients

Glutamine Supplementation Prevents Chronic Stress-Induced Mild Cognitive Impairment.

Authors - Ji Hyeong Baek