NOXA2/p67phox Polyclonal antibody

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  WB analysis using 15551-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15551-1-AP (NOXA2/p67phox antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15551-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15551-1-AP (NOXA2/p67phox antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15551-1-AP (NOXA2/p67phox antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human tonsillitis using 15551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15551-1-AP (NOXA2/p67phox antibody at dilution of 1:50(under 40x lens).

NOXA2/p67phox Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15551-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

NCF2, NOXA2, P67PHOX, NCF 2, NCF-2

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Tested Applications

• Positive WB detected in: U-251 cells, COLO 320 cells, MCF-7 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

15551-1-AP targets NOXA2/p67phox in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7909

  Product name: Recombinant human NCF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 178-526 aa of BC001606

  Sequence: PVGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV

• Full Name: neutrophil cytosolic factor 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC001606
• Gene Symbol: NCF2
• Gene ID (NCBI): 4688
• RRID: AB_11182937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19878
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
human	WB	Redox Biol; Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.; Authors - Ming Kong
mouse	WB,IF	Br J Pharmacol; Rhein regulates redox-mediated activation of NLRP3 inflammasomes in intestinal inflammation through macrophage-activated crosstalk.; Authors - Yangyang Zhou
human	WB	Free Radic Biol Med; S100A8 induces Cyclophosphamide-Induced Alopecia via NCF2/NOX2-Mediated Ferroptosis; Authors - Wen Xu
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang