NOXA2/p67phox Polyclonal antibody

NOXA2/p67phox Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15551-1-AP

Synonyms

NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, NOXA2/p67phox, P67 PHOX, p67phox



Tested Applications

Positive WB detected inU-251 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15551-1-AP targets NOXA2/p67phox in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOXA2/p67phox fusion protein Ag7909
Full Name neutrophil cytosolic factor 2
Calculated molecular weight 60 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC001606
Gene symbol NCF2
Gene ID (NCBI) 4688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NOXA2/p67phox antibody 15551-1-APDownload protocol
IHC protocol for NOXA2/p67phox antibody 15551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

ACS Nano

Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.

Authors - Yiyuan Kang
humanWB

J Hazard Mater

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.

Authors - Feng Xiaoli
humanWB

Redox Biol

Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.

Authors - Ming Kong
mouseWB,IF

Br J Pharmacol

Rhein regulates redox-mediated activation of NLRP3 inflammasomes in intestinal inflammation through macrophage-activated crosstalk.

Authors - Yangyang Zhou
humanIHC

Front Immunol

Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction

Authors - Hang Ji
ratWB

FASEB J

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

Authors - Catherine E Aiken