Tested Applications
| Positive WB detected in | U-937 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10206-1-AP targets NCK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0262 Product name: Recombinant human NCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-235 aa of BC000103 Sequence: IAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWW Predict reactive species |
| Full Name | NCK adaptor protein 2 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC000103 |
| Gene Symbol | NCK2 |
| Gene ID (NCBI) | 8440 |
| RRID | AB_2150571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43639 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NCK2 antibody 10206-1-AP | Download protocol |
| WB protocol for NCK2 antibody 10206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NCK2 Polyclonal antibody (Cat# 10206-1-AP) has 2 total citations. These appear in Biochemical Pharmacology (2024), studying KLF4-mediated suppression of brusatol's anticancer effects via NCK2 upregulation in melanoma, and Evidence-Based Complementary and Alternative Medicine (2020), investigating icariin's alleviation of glucocorticoid-induced osteoporosis through the EphB4/Ephrin-B2 axis. The associated research fields are oncology (melanoma) and bone metabolism (osteoporosis). Both studies used the antibody for Western blot in human and mouse samples, respectively.
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol KLF4 suppresses anticancer effects of brusatol via transcriptional upregulating NCK2 expression in melanoma
| ||
Evid Based Complement Alternat Med Icariin Alleviates Glucocorticoid-Induced Osteoporosis through EphB4/Ephrin-B2 Axis. |
