Product Information
10454-1-AP targets HEM1; NCKAP1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0706 Product name: Recombinant human HEM1; NCKAP1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 968-1127 aa of BC001604 Sequence: VGCDIDPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREVSRAFHLN Predict reactive species |
| Full Name | NCK-associated protein 1-like |
| Calculated Molecular Weight | 128 kDa |
| GenBank Accession Number | BC001604 |
| Gene Symbol | NCKAP1L |
| Gene ID (NCBI) | 3071 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55160 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Membrane-associated protein HEM1 encoded by human NCKAP1L (Nck-associated protein 1-like) gene belongs to the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. HEM1 is characterized as hematopoietic cell-specific actin regulatory protein. HEM1 is a component of the "WAVE" complex, which signals downstream of activated Rac to stimulate F-actin polymerization in response to immuno-receptor signaling. Therefore, HEM1 may be essential for most active processes dependent on reorganization of the actin cytoskeleton in immune cells.
Publications
What published studies show
The HEM1/NCKAP1L polyclonal antibody (Cat# 10454-1-AP) has 1 total citation. It was cited in the journal Gene (2024), in a study investigating the predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis. The research field is oncology, specifically bone cancer (osteosarcoma) and immune-related gene expression. The antibody was used in WB and IHC applications on human samples.
| Species | Application | Title |
|---|---|---|
Gene Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis |
