Nicalin Polyclonal antibody

Nicalin Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18444-1-AP

Synonyms

NCLN, Nicalin, nicalin homolog (zebrafish), Nicastrin like protein

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Validation Data Gallery

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Tested Applications

• Positive WB detected in: SKOV-3 cells
• Positive IF detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18444-1-AP is Nicalin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nicalin fusion protein Ag13318
• Full Name: nicalin homolog (zebrafish)
• Calculated Molecular Weight: 63 kDa
• GenBank Accession Number: BC010064
• Gene Symbol: NCLN
• Gene ID (NCBI): 56926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicalin, also named as NCLN, is distantly related to Nicastrin, a component of the Alzheimer's disease-associated γ-secretase, and forms a complex with Nomo. It also forms a complex with NOMO2 and TMEM147.