NCOA6 Polyclonal antibody

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• KD/KO Validated

NCOA6 Polyclonal Antibody for WB, IP, ELISA

Cat No. 25241-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

AIB3, ANTP, ASC 2, ASC2, ASC-2

• 

  WB analysis of mouse liver using 25241-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25241-1-AP (NCOA6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 25241-1-AP

  IP result of anti-NCOA6 (IP:25241-1-AP, 4ug; Detection:25241-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25241-1-AP targets NCOA6 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18737

  Product name: Recombinant human NCOA6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1715-2060 aa of BC136272

  Sequence: PNALSSSPAPNIQTGRPLVLSSRATPVQLPSPPCTSSPVVPSHPPVQQVKELNPDEASPQVNTSADQNTLPSSQSTTMVSPLLTNSPGSSGNRRSPVSSSKGKGKVDKIGQILLTKACKKVTGSLEKGEEQYGADGETEGQGLDTTAPGLMGTEQLSTELDSKTPTPPAPTLLKMTSSPVGPGTASAGPSLPGGALPTSVRSIVTTLVPSELISAVPTTKSNHGGIASESLAGGLVEEKVGSHPELLPSIAPSQNLVSKETSTTALQASVARPELEVNAAIVSGQSSEPKEIVEKSKIPGRRNSRTEEPTVASESVENGHRKRSSRPASASSSTKDITSAVQSKRR

• Full Name: nuclear receptor coactivator 6
• Calculated Molecular Weight: 2063 aa, 219 kDa
• Observed Molecular Weight: 110 kDa, 220-250 kDa
• GenBank Accession Number: BC136272
• Gene Symbol: NCOA6
• Gene ID (NCBI): 23054
• RRID: AB_2879983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14686
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCOA6 (Nuclear receptor coactivator 6), also named as AIB3 or ANTP, is a 2063 amino acid protein, which contains two LXXLL motifs. NCOA6 localizes in the nucleus and is highly expressed in brain, prostate, testis and ovary. NCOA6 directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion.

Publications

What published studies show

NCOA6 Polyclonal antibody (Cat# 25241-1-AP) has 8 total citations. These appear in Nature Cell Biology, Nature Communications (×2), Cancer Cell International, Molecular Therapy–Nucleic Acids, Drug Metabolism and Disposition, Cancer Medicine, and Molecular Pharmacology. Research fields span cancer biology (breast and pancreatic cancer), epigenetics and chromatin regulation, brown adipose tissue metabolism, trophoblast development in preeclampsia, hepatic drug metabolism, and Hippo signaling in chromosomal instability.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cell Biol; TRACERx analysis identifies a role for FAT1 in regulating chromosomal instability and whole-genome doubling via Hippo signalling; Authors - Wei-Ting Lu
		Nat Commun; The H3K4 methyltransferase KMT2D is an essential cofactor for GATA1 at erythroid gene enhancers.; Authors - Jianxiang Zhang
mouse	WB	Nat Commun; Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue.; Authors - Manuela Sánchez-Feutrie
human	WB	Cancer Cell Int; Phosphorylated NCOA6 promotes aggressiveness and contributes patient outcomes in estrogen receptor-positive breast cancer.; Authors - Hongmei Dong
human	WB	Mol Ther Nucleic Acids; Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE.; Authors - Yetao Xu
		Drug Metab Dispos; Histone Modifications Regulate the Developmental Expression of Human Hepatic UDP-Glucuronosyltransferase 1A1.; Authors - Ya-Li Nie