NCOA6 Polyclonal antibody

• Featured Product
• KD/KO Validated

NCOA6 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25241-1-AP

Synonyms

AIB3, ANTP, ASC 2, ASC2, HOX1.1, HOXA7, KIAA0181, NCOA6, NRC, NRC RAP250, nuclear receptor coactivator 6, PPAR interacting protein, PRIP, RAP250, TRBP

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 25241-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25241-1-AP (NCOA6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 25241-1-AP

  IP result of anti-NCOA6 (IP:25241-1-AP, 4ug; Detection:25241-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

---

Tested Applications

• Positive IP detected in: HEK-293 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

25241-1-AP targets NCOA6 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCOA6 fusion protein Ag18737
• Full Name: nuclear receptor coactivator 6
• Calculated Molecular Weight: 2063 aa, 219 kDa
• Observed Molecular Weight: 68 kDa, 219 kDa, 110 kDa
• GenBank Accession Number: BC136272
• Gene Symbol: NCOA6
• Gene ID (NCBI): 23054
• RRID: AB_2879983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCOA6 (Nuclear receptor coactivator 6), also named as AIB3 or ANTP, is a 2063 amino acid protein, which contains two LXXLL motifs. NCOA6 localizes in the nucleus and is highly expressed in brain, prostate, testis and ovary. NCOA6 directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. The molecular weight of NCOA6 is 219 kDa, but we always detected 68 kDa protein by western blot.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE.; Authors - Yetao Xu
human	WB,IF	Mol Pharmacol; Alterations of Histone Modifications Contribute to Pregnane X Receptor-mediated Induction of CYP3A4 by Rifampicin.; Authors - Liang Yan
human	IHC,CoIP	Cancer Med; Nuclear receptor coactivator 6 (NCoA6) promotes cell proliferation, migration, and invasion in pancreatic cancer; Authors - Xin Wang ; KD Validated