NCOA6 Recombinant monoclonal antibody

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• NCOA6 Recombinant monoclonal antibody (87454-1-RR)

• 

  WB analysis using 87454-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87454-1-RR (NCOA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity of 87454-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87454-1-RR against Human NCOA6 were performed. The affinity constant is 3.26 nM.

NCOA6 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87454-1-RR

Clone No.252826D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AIB3, ANTP, ASC 2, ASC2, ASC-2

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Tested Applications

• Positive WB detected in: HEK-293 cells, PANC-1 cells, SW 1990 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87454-1-RR targets NCOA6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18737

  Product name: Recombinant human NCOA6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1715-2060 aa of BC136272

  Sequence: PNALSSSPAPNIQTGRPLVLSSRATPVQLPSPPCTSSPVVPSHPPVQQVKELNPDEASPQVNTSADQNTLPSSQSTTMVSPLLTNSPGSSGNRRSPVSSSKGKGKVDKIGQILLTKACKKVTGSLEKGEEQYGADGETEGQGLDTTAPGLMGTEQLSTELDSKTPTPPAPTLLKMTSSPVGPGTASAGPSLPGGALPTSVRSIVTTLVPSELISAVPTTKSNHGGIASESLAGGLVEEKVGSHPELLPSIAPSQNLVSKETSTTALQASVARPELEVNAAIVSGQSSEPKEIVEKSKIPGRRNSRTEEPTVASESVENGHRKRSSRPASASSSTKDITSAVQSKRR

• Full Name: nuclear receptor coactivator 6
• Calculated Molecular Weight: 2063 aa, 219 kDa
• Observed Molecular Weight: 220-250 kDa
• GenBank Accession Number: BC136272
• Gene Symbol: NCOA6
• Gene ID (NCBI): 23054
• RRID: AB_3745558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14686
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCOA6 (Nuclear receptor coactivator 6), also named as AIB3 or ANTP, is a 2063 amino acid protein, which contains two LXXLL motifs. NCOA6 localizes in the nucleus and is highly expressed in brain, prostate, testis and ovary. NCOA6 directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion.