NCOA7 Polyclonal antibody

• Featured Product
• KD/KO Validated

NCOA7 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23092-1-AP

Synonyms

ERAP140, ESNA1, Nbla00052, Nbla10993, NCOA7, nuclear receptor coactivator 7

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Validation Data Gallery

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  WB analysis of HEK-293 using 23092-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23092-1-AP (NCOA7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23092-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23092-1-AP (NCOA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23092-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23092-1-AP (NCOA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23092-1-AP

  IP result of anti-NCOA7 (IP:23092-1-AP, 4ug; Detection:23092-1-AP 1:1000) with HEK-293 cells lysate 3600ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 23092-1-AP is NCOA7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCOA7 fusion protein Ag19315
• Full Name: nuclear receptor coactivator 7
• Calculated Molecular Weight: 972 aa, 106 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC137094
• Gene Symbol: NCOA7
• Gene ID (NCBI): 135112
• RRID: AB_2879208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. NCoA-7 (nuclear receptor coactivator 7), also known as ESNA1 or ERAP140, is a 942 amino acid nuclear protein that enhances nuclear receptor transcriptional activities and coactivates several nuclear receptors including PPARG, ESR1, THRB and RARA. Highly expressed in brain and weakly expressed in pancreas, bladder, ovary, spinal cord, prostate, mammary gland, ovary, uterus and stomach, NCoA-7 is a member of the OXR1 family and contains one LysM repeat and a TLD domain. This antibody is a rabbit polyclonal antibody raised against the N-terminal of human NCOA7 protein.

Publications

Species	Application	Title
mouse	WB,CoIP	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio ; KO Validated
		Am J Physiol Renal Physiol; Targeted Deletion of the Ncoa7 Gene Results in Incomplete Distal Renal Tubular Acidosis in Mice.; Authors - Maria Merkulova
human	WB,IHC,IF	Int J Mol Sci; NCOA7 Regulates Growth and Metastasis of Clear Cell Renal Cell Carcinoma via MAPK/ERK Signaling Pathway; Authors - Jiayu Guo