Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20018-1-AP targets NCOR1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nuclear receptor co-repressor 1 |
| Calculated Molecular Weight | 270 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | NM_006311 |
| Gene Symbol | NCOR1 |
| Gene ID (NCBI) | 9611 |
| RRID | AB_10665360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75376 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NCOR1, also named as KIAA1047 and TRAC1, belongs to the N-CoR nuclear receptor corepressors family. NCO1 mediates transcriptional repression by certain nuclear receptors. It is part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. The antibody recognizes the 103 kDa isoform of NCOR1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NCOR1 antibody 20018-1-AP | Download protocol |
| WB protocol for NCOR1 antibody 20018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody 20018-1-AP (anti-NCoR1) has 10 total citations published in journals including Cell, Cell Metabolism, Nature Communications, Redox Biology, Theranostics, Phytotherapy Research, PLoS Biology, Cellular and Molecular Life Sciences, Geroscience, and iScience. Research fields span tumor immunology, metabolic regulation, non-alcoholic fatty liver disease, ferroptosis in PCOS, hepatic steatosis and autophagy, circadian lipid metabolism, spatial memory impairment, aging-related oxidative metabolism, and diabetic cardiac fibrosis.
| Species | Application | Title |
|---|---|---|
Cell Osr2 functions as a biomechanical checkpoint to aggravate CD8+ T cell exhaustion in tumor | ||
Cell Metab Methionine-supplemented longevity diet increases growth hormone, GLP-1, and FGF21; reduces frailty; and promotes healthspan. | ||
Nat Commun Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease | ||
Redox Biol Methylation reader MBD2-mediated GPX4 transcriptional repression drives ovarian granulosa cell ferroptosis in PCOS. | ||
Theranostics Palmitoylation of PKCδ by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver disease | ||
Phytother Res Scutellarin Alleviates Hepatic Steatosis by Autophagy-Mediated NCoR1/PPARα-Driven Fatty Acid β-Oxidation and Enhancing Peroxisome Biogenesis. |
