NCOR2 Polyclonal antibody

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  WB analysis using 29952-1-AP

  MOLT-4 cells were subjected to Tris-Acetate gel system followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29952-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 29952-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 29952-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 29952-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NCOR2 antibody (29952-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NCOR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 29952-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CTG repeat protein 26, CTG26, N CoR2, NCOR2, Nuclear receptor corepressor 2, SMAP270, SMRT, SMRTE, SMRTE tau, T3 receptor associating factor, TNRC14, TRAC, TRAC 1, TRAC1

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29952-1-AP targets NCOR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30727

  Product name: Recombinant human NCOR2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1165-1363 aa of NM_006312

  Sequence: SGVKQEQLSPRGQAGPPESLGVPTAQEASVLRGTALGSVPGGSITKGIPSTRVPSDSAITYRGSITHGTPADVLYKGTITRIIGEDSPSRLDRGREDSLPKGHVIYEGKKGHVLSYEGGMSVTQCSKEDGRSSSGPPHETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHHLKEQHHIRGSITQGIPRSY

• Full Name: nuclear receptor co-repressor 2
• Calculated Molecular Weight: 274 kDa
• Observed Molecular Weight: 274 kDa
• GenBank Accession Number: NM_006312
• Gene Symbol: NCOR2
• Gene ID (NCBI): 9612
• RRID: AB_3086197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y618
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3-Regulated Histone Deacetylation and Transcription; Authors - Chen-Hui Li
mouse	WB	Eur J Med Chem; Design, synthesis and bioevaluation of novel hydrazide derivatives as enhancers of immunotherapy and DNA-damage response in antitumor therapy; Authors - Zhihao Hu