Tested Applications
| Positive WB detected in | Jurkat cells, MOLT-4 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29952-1-AP targets NCOR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30727 Product name: Recombinant human NCOR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1165-1363 aa of NM_006312 Sequence: SGVKQEQLSPRGQAGPPESLGVPTAQEASVLRGTALGSVPGGSITKGIPSTRVPSDSAITYRGSITHGTPADVLYKGTITRIIGEDSPSRLDRGREDSLPKGHVIYEGKKGHVLSYEGGMSVTQCSKEDGRSSSGPPHETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHHLKEQHHIRGSITQGIPRSY Predict reactive species |
| Full Name | nuclear receptor co-repressor 2 |
| Calculated Molecular Weight | 274 kDa |
| Observed Molecular Weight | 274 kDa |
| GenBank Accession Number | NM_006312 |
| Gene Symbol | NCOR2 |
| Gene ID (NCBI) | 9612 |
| RRID | AB_3086197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y618 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NCOR2 antibody 29952-1-AP | Download protocol |
| IHC protocol for NCOR2 antibody 29952-1-AP | Download protocol |
| WB protocol for NCOR2 antibody 29952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NCOR2 Polyclonal antibody (Cat# 29952-1-AP) has 3 citations published in Advanced Science, European Journal of Medicinal Chemistry, and BMC Pregnancy and Childbirth. The associated research fields include epigenetic transcription regulation and spliceosomal interactions, immunotherapy enhancement and DNA-damage response in antitumor therapy, and Notch pathway-related protein expression in preeclampsia. All citations used Western blot as the application, with reactivity in human and mouse species.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3-Regulated Histone Deacetylation and Transcription | ||
Eur J Med Chem Design, synthesis and bioevaluation of novel hydrazide derivatives as enhancers of immunotherapy and DNA-damage response in antitumor therapy | ||
BMC Pregnancy Childbirth Altered Notch pathway-related protein expression in cord blood of preeclampsia offspring: a pilot study. |
