Nicastrin Polyclonal antibody

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• KD/KO Validated

Nicastrin Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14071-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ1874/PRO4317, NCSTN, KIAA0253

• 

  WB analysis of mouse brain using 14071-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of C6 using 14071-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14071-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of C6 using 14071-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using Nicastrin antibody (14071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, C6 cells, human brain tissue
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14071-1-AP targets Nicastrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5137

  Product name: Recombinant human Nicastrin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 338-689 aa of BC047621

  Sequence: NVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

• Full Name: nicastrin
• Calculated Molecular Weight: 709 aa, 78.4 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC047621
• Gene Symbol: Nicastrin
• Gene ID (NCBI): 23385
• RRID: AB_2149583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92542
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

Publications

What published studies show

Nicastrin polyclonal antibody (Cat# 14071-1-AP) has 8 total citations published in Gut Microbes, J Neuroinflammation, Aging Cell, Cancer Cell Int, Mol Neurobiol, Front Oncol, Neurosci Lett, and Heliyon. Research fields span Alzheimer's disease pathogenesis and Aβ clearance, cerebral amyloid angiopathy, hepatocellular carcinoma, metformin therapeutic response, and moyamoya disease biomarker identification.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Gut Microbes; Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis; Authors - Jinghua Jin
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
mouse	WB	Aging Cell; Myeloid ectopic viral integration site 2 accelerates the progression of Alzheimer's disease; Authors - Yuting Cui
human	WB,IHC	Cancer Cell Int; Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation.; Authors - Xicheng Wang ; KD Validated
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue
human	WB	Front Oncol; Stimulation of Let-7 Maturation by Metformin Improved the Response to Tyrosine Kinase Inhibitor Therapy in an m6A Dependent Manner.; Authors - Kai Li