Nicastrin Polyclonal antibody

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  WB analysis of mouse brain using 14071-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 14071-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14071-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of C6 using 14071-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using Nicastrin antibody (14071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

Nicastrin Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14071-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ1874/PRO4317, NCSTN, KIAA0253

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Tested Applications

• Positive WB detected in: mouse brain tissue, C6 cells, human brain tissue
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

14071-1-AP targets Nicastrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5137

  Product name: Recombinant human Nicastrin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 338-689 aa of BC047621

  Sequence: NVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

• Full Name: nicastrin
• Calculated Molecular Weight: 709 aa, 78.4 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC047621
• Gene Symbol: Nicastrin
• Gene ID (NCBI): 23385
• RRID: AB_2149583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92542
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Gut Microbes; Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis; Authors - Jinghua Jin
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
human	WB	Front Oncol; Stimulation of Let-7 Maturation by Metformin Improved the Response to Tyrosine Kinase Inhibitor Therapy in an m6A Dependent Manner.; Authors - Kai Li
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue
human	WB,IHC	Cancer Cell Int; Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation.; Authors - Xicheng Wang ; KD Validated
mouse	WB	Neurosci Lett; Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.; Authors - Yasuteru Inoue