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  • KD/KO Validated

ND1 Polyclonal antibody

ND1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 19703-1-AP

Synonyms

MT-ND1, NADH1, MTND1, MT ND1



Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, mouse heart tissue, mouse kidney tissue, mouse liver mitochondria
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19703-1-AP targets ND1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name NADH dehydrogenase, subunit 1 (complex I)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 28-38 kDa
GenBank Accession NumberNC_012920
Gene Symbol ND1
Gene ID (NCBI) 4535
RRIDAB_10637853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND1 belongs to the complex I subunit 1 family. ND1 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Protocols

Product Specific Protocols
WB protocol for ND1 antibody 19703-1-APDownload protocol
IHC protocol for ND1 antibody 19703-1-APDownload protocol
IP protocol for ND1 antibody 19703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Single-Cell Transcriptomic Atlas of Primate Ovarian Aging.

Authors - Si Wang
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
  • KD Validated
mouseIHC

Circulation

Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.

Authors - Jorge Oller
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Sci Adv

MCART1/SLC25A51 is required for mitochondrial NAD transport.

Authors - Nora Kory
mouseWB

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tania (Verified Customer) (03-05-2022)

a lot of background. difficult to say each band is the correct one.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ES2 human cells
FH

Daniel (Verified Customer) (10-01-2019)

ND2 was detected in both liver (L) and heart (H) lysate from a wildtype mouse. No ND1 was detected in samples processed in parallel.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver (L), heart (H)
ND1 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (L), heart (H) (Cat no:19703-1-AP)
FH

Loukmane (Verified Customer) (08-10-2018)

It works very well with A549 and lung tissue, although with the former the background is higherThe image shows ND1 in whole A549 lysate

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Lung tissue and A549
ND1 Antibody Western Blot, validation (1:500 dilution) in Lung tissue and A549 (Cat no:19703-1-AP)