Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue, mouse kidney tissue, human kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55410-1-AP targets MT-ND5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NADH dehydrogenase, subunit 5 (complex I) |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | AF465942 |
| Gene Symbol | MT-ND5 |
| Gene ID (NCBI) | 4540 |
| RRID | AB_2881324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P03915 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MT-ND5 antibody 55410-1-AP | Download protocol |
| WB protocol for MT-ND5 antibody 55410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MT-ND5 Polyclonal antibody (Cat# 55410-1-AP) has accumulated 47 citations in high-impact journals including Nature, Science, Cell, Cell Metabolism, Molecular Cell, EMBO Journal, Nature Metabolism, Cell Death & Disease, and iScience. Key research fields encompass mitochondrial translation, oxidative phosphorylation, cancer metabolic reprogramming, neurodegeneration, mitochondrial biogenesis, and mitochondrial DNA maintenance. The antibody is predominantly applied in Western blot across human, mouse, rat, and other species.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes | ||
Cancer Cell Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma. | ||
Science Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice | ||
Cell PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma | ||
Cell Dynamic mapping of proteome trafficking within and between living cells by TransitID |
Reviews
What customers say
Both reviews are for Western Blot applications. One user (3/5) reported the antibody performed adequately on bladder cancer cells. The other (4/5) used a 1:500 dilution on lymphoblastoid cells and found it working well, though they noted a faint non-specific band near the expected molecular weight. Overall, the antibody is considered functional for WB with minor non-specific binding observed in one case.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Prakash (Verified Customer) (10-28-2025) | its OK in western blot
|
Baptiste (Verified Customer) (05-23-2025) | Working well but have a light unspecific (?) band close to ND5.
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