ND5 Monoclonal antibody

ND5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1C12G8

Cat no : 66613-1-Ig

Synonyms

MT ND5, MTND5, NADH dehydrogenase subunit 5, NADH5, ND5

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Validation Data Gallery

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  WB analysis using 66613-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66613-1-Ig (ND5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, L02 cells, HepG2 cells, COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66613-1-Ig targets ND5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NADH dehydrogenase, subunit 5 (complex I)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: AF465942
• Gene Symbol: ND5
• Gene ID (NCBI): 4540
• RRID: AB_2881973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Rep; LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1.; Authors - Xiaoting Liu