NDC80 Monoclonal antibody

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  WB analysis using 66960-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66960-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66960-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

NDC80 Monoclonal Antibody for WB, ELISA

Cat No. 66960-1-Ig

Clone No.2B7A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

2B7A8, HEC, HEC1, HsHec1, hsNDC80

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Tested Applications

• Positive WB detected in: HeLa cells, NCCIT cells, HSC-T6 cells, LNCaP cells, Jurkat cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66960-1-Ig targets NDC80 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8065

  Product name: Recombinant human NDC80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 290-642 aa of BC035617

  Sequence: PNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

• Full Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 71-74 kDa
• GenBank Accession Number: BC035617
• Gene Symbol: NDC80
• Gene ID (NCBI): 10403
• RRID: AB_2882283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14777
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.