HEC1/HEC/NDC80 Monoclonal antibody

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• HEC1/HEC/NDC80 Monoclonal antibody (66960-1-Ig)

HEC1/HEC/NDC80 Monoclonal Antibody for WB, ELISA

Cat No. 66960-1-Ig Copied!

Clone No. 2B7A8

Publications (2)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NDC80, 2B7A8, HEC, HEC1, HsHec1

• 

  WB analysis using 66960-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66960-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HSC-T6 using 66960-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66960-1-Ig (NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, NCCIT cells, HSC-T6 cells, LNCaP cells, Jurkat cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66960-1-Ig targets HEC1/HEC/NDC80 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8065

  Product name: Recombinant human NDC80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 290-642 aa of BC035617

  Sequence: PNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

• Full Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 71-74 kDa
• GenBank Accession Number: BC035617
• Gene Symbol: NDC80
• Gene ID (NCBI): 10403
• RRID: AB_2882283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14777
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The HEC1/HEC/NDC80 Monoclonal antibody (2B7A8), catalog number 66960-1-Ig, has 2 total citations. Both appear in Science Advances and bioRxiv (preprint of the same study). The research focuses on transcriptional readthrough and alternative splicing programs in chronic myeloid leukemia (CML) cells treated with imatinib, placing it within the fields of cancer biology, RNA processing, and targeted therapy response.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.; Authors - Paulina Podszywałow-Bartnicka
human	WB	bioRxiv; Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.; Authors - Paulina Podszywałow-Bartnicka