NDC80 Monoclonal antibody

NDC80 Monoclonal Antibody for WB, ELISA

Cat No. 66960-1-Ig
Clone No.2B7A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

2B7A8, HEC, HEC1, HsHec1, hsNDC80

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, NCCIT cells, HSC-T6 cells, LNCaP cells, Jurkat cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66960-1-Ig targets NDC80 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8065

Product name: Recombinant human NDC80 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 290-642 aa of BC035617

Sequence: PNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

Predict reactive species
Full Name NDC80 homolog, kinetochore complex component (S. cerevisiae)
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 71-74 kDa
GenBank Accession NumberBC035617
Gene Symbol NDC80
Gene ID (NCBI) 10403
RRIDAB_2882283
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO14777
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDC80 antibody 66960-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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