Tested Applications
Positive WB detected in | HeLa cells, NCCIT cells, HSC-T6 cells, LNCaP cells, Jurkat cells, THP-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66960-1-Ig targets NDC80 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen |
CatNo: Ag8065 Product name: Recombinant human NDC80 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 290-642 aa of BC035617 Sequence: PNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE Predict reactive species |
Full Name | NDC80 homolog, kinetochore complex component (S. cerevisiae) |
Calculated Molecular Weight | 74 kDa |
Observed Molecular Weight | 71-74 kDa |
GenBank Accession Number | BC035617 |
Gene Symbol | NDC80 |
Gene ID (NCBI) | 10403 |
RRID | AB_2882283 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O14777 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for NDC80 antibody 66960-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |