NDP Polyclonal antibody

NDP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15380-1-AP

Synonyms

EVR2, FEVR, ND, NDP, Norrie disease (pseudoglioma), Norrie disease protein, Norrin

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Validation Data Gallery

• 

  WB analysis of mouse testis using 15380-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15380-1-AP (NDP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 15380-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 15380-1-AP (NDP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 15380-1-AP is NDP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDP fusion protein Ag4066
• Full Name: Norrie disease (pseudoglioma)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC029901
• Gene Symbol: NDP
• Gene ID (NCBI): 4693
• RRID: AB_2878133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDP, also named as EVR2, ND and Norrin, is a secreted regulatory protein that remains tightly associated with the extracellular matrix. Mutations in the NDP gene are associated with the Norrie disease. Signaling induced by the protein NDP regulates vascular development of vertebrate retina and controls important blood vessels in the ear. It binds with high affinity to Frizzled 4, and Frizzled 4 knockout mice exhibit abnormal vascular development of the retina. This antibody detects 11-15 kDa (monomer) and 48 kDa (polymers, refer: 25692504).