NDRG2 Polyclonal antibody

NDRG2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12015-1-AP

Synonyms

KIAA1248, NDRG family member 2, NDRG2, Protein NDRG2, Protein Syld709613, SYLD

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Validation Data Gallery

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  WB analysis of rat brain using 12015-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12015-1-AP (NDRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12015-1-AP (NDRG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 12015-1-AP

  IP result of anti-NDRG2 (IP:12015-1-AP, 4ug; Detection:12015-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 12015-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12015-1-AP (NDRG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12015-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12015-1-AP (NDRG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12015-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12015-1-AP (NDRG2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12015-1-AP targets NDRG2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDRG2 fusion protein Ag2629
• Full Name: NDRG family member 2
• Calculated Molecular Weight: 371 aa, 39 kDa
• Observed Molecular Weight: 41-43 kDa
• GenBank Accession Number: BC010458
• Gene Symbol: NDRG2
• Gene ID (NCBI): 57447
• RRID: AB_10666856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Ovarian Res; NDRG2 gene expression pattern in ovarian cancer and its specific roles in inhibiting cancer cell proliferation and suppressing cancer cell apoptosis.; Authors - Fenhong Kang