NDRG4 Polyclonal antibody

NDRG4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12184-1-AP

Synonyms

SMAP 8, Protein NDRG4, N-myc downstream-regulated gene 4 protein, NDRG family member 4, Brain development-related molecule 1

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Validation Data Gallery

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  WB analysis of human brain using 12184-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12184-1-AP (NDRG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12184-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12184-1-AP (NDRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human medulloblastoma using 12184-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human medulloblastoma using 12184-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 12184-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 12184-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 12184-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 12184-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12184-1-AP (NDRG4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 12184-1-AP

  Immunofluorescent analysis of HepG2 cells, using NDRG4 antibody 12184-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse kidney tissue
• Positive IHC detected in: human medulloblastoma tissue, human colon tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12184-1-AP targets NDRG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDRG4 fusion protein Ag3029
• Full Name: NDRG family member 4
• Calculated Molecular Weight: 339 aa, 37 kDa
• Observed Molecular Weight: 37 kDa, 38 kDa and 41 kDa
• GenBank Accession Number: BC011795
• Gene Symbol: NDRG4
• Gene ID (NCBI): 65009
• RRID: AB_2235852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	DNA Cell Biol; The Role of N-myc Downstream-Regulated Gene Family in Glioma Based on Bioinformatics Analysis.; Authors - Ting Tang
human	WB	BMB Rep; Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function.; Authors - Hao-Hong Shi
		Cell Rep; The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.; Authors - Brian P Hermann