NDST1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDST1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 26203-1-AP

Synonyms

HSST, HSST1, N HSST 1, NDST 1, NDST1, NST1

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Validation Data Gallery

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  WB analysis using 26203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26203-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 26203-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 26203-1-AP

  IP result of anti-NDST1 (IP:26203-1-AP, 4ug; Detection:26203-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse liver tissue, mouse heart tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

26203-1-AP targets NDST1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDST1 fusion protein Ag24422
• Full Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• Calculated Molecular Weight: 882 aa, 101 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC012888
• Gene Symbol: NDST1
• Gene ID (NCBI): 3340
• RRID: AB_2880423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	WB,IF	Ann Neurol; Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice.; Authors - Yang Huang ; KD Validated
human	WB	PLoS Pathog; N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells.; Authors - Nan Song ; KO Validated
human	WB	Matrix Biol Plus; Glycocalyx mechanotransduction mechanisms are involved in renal cancer metastasis.; Authors - Heriberto Moran