NDUFA10 Polyclonal antibody

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• NDUFA10 Polyclonal antibody (32947-1-AP)

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  WB analysis using 32947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 32947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 32947-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 32947-1-AP (NDUFA10 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 32947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 32947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NDUFA10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 32947-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CI-42kD, Complex I-42kD, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:Ubiquinone Oxidoreductase Subunit A10, NADH-ubiquinone oxidoreductase 42 kDa subunit

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells, RAW264.7 cells, mouse liver tissue, Jurkat cells, RAW 264.7 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32947-1-AP targets NDUFA10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37576

  Product name: Recombinant human NDUFA10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-355 aa of BC003417

  Sequence: MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK

• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC003417
• Gene Symbol: NDUFA10
• Gene ID (NCBI): 4705
• RRID: AB_3742801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95299
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFA10 (NADH:ubiquinone oxidoreductase subunit A10) is a subunit of mitochondrial complex I of the electron transport chain, encoding a protein with NADH dehydrogenase and oxidoreductase activity. This protein transfers electrons from NADH to the respiratory chain. Mutations in the NDUFA10 gene are associated with Leigh syndrome, a common mitochondrial energy metabolism disorder in children. Additionally, the expression levels of NDUFA10 are related to the occurrence and prognosis of various diseases. For example, in Alzheimer's disease, its low expression is associated with increased dementia severity. In prostate cancer, low expression of NDUFA10 predicts a poor prognosis.