GRIM19 Polyclonal antibody

• Featured Product
• KD/KO Validated

GRIM19 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10986-1-AP

Synonyms

B16.6, CDA016, CGI 39, CI B16.6, Complex I B16.6, GRIM 19, GRIM19, NDUFA13

---

Validation Data Gallery

• 

  WB analysis using 10986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10986-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10986-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10986-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10986-1-AP

  IP result of anti-GRIM19 (IP:10986-1-AP, 4ug; Detection:10986-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human prostate cancer using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, HEK-293 cells, MCF-7 cells, rat heart tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue, human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below

Product Information

10986-1-AP targets GRIM19 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRIM19 fusion protein Ag1435
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
• Calculated Molecular Weight: 16.5 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC009189
• Gene Symbol: GRIM19
• Gene ID (NCBI): 51079
• RRID: AB_2150609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).

Publications

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
mouse,rat	WB	Cell Mol Life Sci; SGK3 deficiency in macrophages suppresses angiotensin II-induced cardiac remodeling via regulating Ndufa13-mediated mitochondrial oxidative stress; Authors - Jiayu Ren
human	WB,IHC	Hum Gene Ther; Nonviral delivery of GRIM-19 gene inhibits tumor growth with reduced local and systemic complications.; Authors - Yang Xu
human	WB,IHC	Exp Cell Res; GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis.; Authors - Ding Wang ; KD Validated
human	WB,IHC	Cancer Chemother Pharmacol; PMID: 30032449; Authors - Feng Ni
		Int J Oncol; Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling.; Authors - Kebang Hu