NDUFAF1 Polyclonal antibody

NDUFAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15181-1-AP

Synonyms

Complex I intermediate-associated protein 30, mitochondrial, CIA30, CGI-65, CGI65, CGI 65

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 15181-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 15181-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15181-1-AP (NDUFAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 15181-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15181-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15181-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15181-1-AP (NDUFAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, Transfected HEK-293 cells, HeLa cells
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15181-1-AP targets NDUFAF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFAF1 fusion protein Ag7162
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000780
• Gene Symbol: NDUFAF1
• Gene ID (NCBI): 51103
• RRID: AB_2150778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Stem Cell Res Ther; Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension.; Authors - Wenda Xi
mouse	WB	Sci Rep; Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.; Authors - David W Reid