Tested Applications
| Positive WB detected in | HEK-293 cells, MDA-MB-453s cells, K-562 cells |
| Positive IHC detected in | human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26003-1-AP targets NDUFAF4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23365 Product name: Recombinant human NDUFAF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC039464 Sequence: MGALVIRGIRNFNLENRAEREISKMKPSVAPRHPSTNSLLREQISLYPEVKGEIARKDEKLLSFLKDVYVDSKDPVSSLQVKAAETCQEPKEFRLPKDHHFDMINIKSIPKGKISIVEALTLLNNHKLFPETWTAEKIMQEYQLEQKDVNSLLKYFVTFEVEIFPPEDKKAIRSK Predict reactive species |
| Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC039464 |
| Gene Symbol | NDUFAF4 |
| Gene ID (NCBI) | 29078 |
| RRID | AB_2880329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P032 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDUFAF4 is a protein that in humans is encoded by the C6ORF66 gene. NDUFAF4 is an assembly factor of complex I. Mutation of this gene has been associated with congenital disorders of the mitochondrial respiratory chain (PMID: 18179882). NDUFAF4 was previously shown to promote breast cancer cell invasiveness. NDUFAF4 is a 20.2 kDa mitochondrial protein. Catalog#26003-1-AP specifically recognises the 20 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFAF4 antibody 26003-1-AP | Download protocol |
| WB protocol for NDUFAF4 antibody 26003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDUFAF4 Polyclonal antibody (Cat# 26003-1-AP) has 6 citations published in Molecular Cell, Cell Death & Disease, Stem Cell Research & Therapy, EMBO Reports, Cancer Research Communications, and Virus Genes. Research fields span mitochondrial quality control, m6A-mediated energy metabolism, stem cell differentiation and hypertension, mitochondrial translation, lung carcinoma with mitochondrial iron overload, and viral propagation. Applications include Western blotting and immunofluorescence in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Cell Death Dis The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR | ||
Stem Cell Res Ther Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension. | ||
EMBO Rep MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex. | ||
Cancer Res Commun N-Myristoyltransferase Inhibition causes Mitochondrial Iron Overload and Parthanatos in TIM17A-dependent Aggressive Lung Carcinoma | ||
Virus Genes The host cellular protein Ndufaf4 interacts with the vesicular stomatitis virus M protein and affects viral propagation.
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