NDUFB1 Polyclonal antibody

NDUFB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16902-1-AP

Synonyms

CI MNLL, CI SGDH, Complex I MNLL, MNLL, NDUFB1

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Validation Data Gallery

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  WB analysis of A375 using 16902-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 16902-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16902-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16902-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16902-1-AP (NDUFB1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16902-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16902-1-AP (NDUFB1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A375 cells, mouse heart tissue, human colon tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16902-1-AP targets NDUFB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB1 fusion protein Ag10443
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa
• Calculated Molecular Weight: 12 kDa, 7 kDa
• Observed Molecular Weight: 7 kDa
• GenBank Accession Number: BC009691
• Gene Symbol: NDUFB1
• Gene ID (NCBI): 4707
• RRID: AB_2150787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.; Authors - Yanchun Ji
	WB	Int J Neurosci; Dysferlinopathy: Mitochondrial Abnormalities in Human Skeletal Muscle.; Authors - Fuchen Liu
human	WB	Cell Rep; A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.; Authors - Hezhi Fang