NDUFB3 Polyclonal antibody

NDUFB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12358-1-AP

Synonyms

B12, CI B12, Complex I B12, NDUFB3

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Validation Data Gallery

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  WB analysis of human heart using 12358-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12358-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 12358-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12358-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12358-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12358-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12358-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human heart tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12358-1-AP targets NDUFB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB3 fusion protein Ag3022
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
• Calculated Molecular Weight: 98 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC018183
• Gene Symbol: NDUFB3
• Gene ID (NCBI): 4709
• RRID: AB_2282633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFB3(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3) is also named as CI-B12 and belongs to the complex I NDUFB3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; Maternal exposure to E 551 during pregnancy leads to genome-wide DNA methylation changes and metabolic disorders in the livers of pregnant mice and their fetuses; Authors - Yingqi Zhan