Tested Applications
| Positive WB detected in | HEK-293 cells, mouse spleen tissue, Jurkat cells, K-562 cells, MCF-7 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
23855-1-AP targets NDUFB5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20907 Product name: Recombinant human NDUFB5 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-189 aa of BC005271 Sequence: MAAMSLLRRVSVTAVAALSGRPLGTRLGFGGFLTRGFPKAAAPVRHSGDHGKRLFVIRPSRFYDRRFLKLLRFYIALTGIPVAIFITLVNVFIGQAELAEIPEGYVPEHWEYYKHPISRWIARNFYDSPEKIYERTMAVLQIEAEKAELRVKELEVRKLMHVRGDGPWYYYETIDKELIDHSPKATPDN Predict reactive species |
| Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa |
| Calculated Molecular Weight | 189 aa, 22 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC005271 |
| Gene Symbol | NDUFB5 |
| Gene ID (NCBI) | 4711 |
| RRID | AB_2879342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O43674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDUFB5 (NADH: Ubiquinone Oxidoreductase Subunit B5) is a 16 kDa accessory subunit of mitochondrial respiratory chain Complex I (NADH: ubiquinone oxidoreductase) in humans. NDUFB5 is a non-catalytic accessory subunit of mitochondrial Complex I essential for proper assembly and function of the respiratory chain. It is ubiquitously expressed, highly conserved across species, and anchored in the mitochondrial inner membrane. While not directly involved in catalysis, it plays a structural and regulatory role in Complex I integrity, and its dysfunction may contribute to mitochondrial diseases and certain cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFB5 antibody 23855-1-AP | Download protocol |
| WB protocol for NDUFB5 antibody 23855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proteome Sci Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells | ||
Basic Res Cardiol Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat |
