NDUFB5 Polyclonal antibody

NDUFB5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23855-1-AP

Synonyms

CI SGDH, Complex I SGDH, NDUFB5, SGDH

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Validation Data Gallery

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  WB analysis of mouse liver using 23855-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 23855-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 23855-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 23855-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse spleen tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 23855-1-AP is NDUFB5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB5 fusion protein Ag20907
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
• Calculated Molecular Weight: 189 aa, 22 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC005271
• Gene Symbol: NDUFB5
• Gene ID (NCBI): 4711
• RRID: AB_2879342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Proteome Sci; Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells; Authors - Xiaowen Xu
rat	WB	Basic Res Cardiol; Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat; Authors - Ling Li