NDUFC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDUFC1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23842-1-AP

Synonyms

NADH-ubiquinone oxidoreductase KFYI subunit, KFYI, Complex I-KFYI, Complex I KFYI, CI-KFYI

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Validation Data Gallery

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  IHC staining of human heart using 23842-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23842-1-AP (NDUFC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 23842-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23842-1-AP (NDUFC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 23842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23842-1-AP (NDUFC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 23842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23842-1-AP (NDUFC1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

23842-1-AP targets NDUFC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFC1 fusion protein Ag20835
• Full Name: NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa
• Calculated Molecular Weight: 76 aa, 9 kDa
• GenBank Accession Number: BC107682
• Gene Symbol: NDUFC1
• Gene ID (NCBI): 4717
• RRID: AB_2879336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; Endocrine persistence in ER+ breast cancer is accompanied by metabolic vulnerability in oxidative phosphorylation; Authors - Steven Tau ; KO Validated